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KMID : 0368419850280020141
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Journal of Plant Biology
1985 Volume.28 No. 2 p.141 ~ p.148
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Effects of Cell Size, Density, Conditioned Media and pH on Carrot(Daucus carota L.) Cell Embryogenesis
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Abstract
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The effects of sizes and densities of cells cultured, conditioned medium, and media pH on the somatic embryogenesis of carrot (Daucus carota L.) were examined. A large number of globular embryoids was formed after 4 days in cell culture, and later globular embryoids developed into heart and torpedo shape. High cell density resulted in higher number and better growth of embryos, especially on conditioned medium than Murashige-Skoog medium. The fresh weight and number of embryoids formed increased with the decrease in cell size. The significant reduction in fresh weight and number of embryoids was obtained when culturing cells with diameter of over 90 ㎛. Dry weight and number of embryoids were markedly reduced with medium pH of 4 or 7, but promoted with pH 6.0.
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